Intraspecific Epitopic Variation in a Carbohydrate Antigen Exposed on the Surface of Trichostrongylus colubriformis Infective L3 Larvae

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Intraspecific Epitopic Variation in a Carbohydrate Antigen Exposed on the Surface of Trichostrongylus colubriformis Infective L3 Larvae

The carbohydrate larval antigen, CarLA, is present on the exposed surface of all strongylid nematode infective L3 larvae tested, and antibodies against CarLA can promote rapid immune rejection of incoming Trichostrongylus colubriformis larvae in sheep. A library of ovine recombinant single chain Fv (scFv) antibody fragments, displayed on phage, was prepared from B cell mRNA of field-immune shee...

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A comparison of the infectivity of cryopreserved versus unfrozen infective larvae of Haemonchus contortus, Trichostrongylus colubriformis

VANWYK, J.A. 1999. A comparison of the infectivity of cryopreserved versus unfrozen infective larvae of Haemonchus contortus, Trichostrongylus colubriformis and Trichostrongylus axei: Results of the Onderstepoort Veterinary Institute from 1977 to the present. Onderstepoort Journal of Veterinary Research , 66:285289 The infectivity for sheep of cryopreserved infective larvae (L3) of various stra...

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Cryopreservation of Infective Third-Stage Larvae of Trichostrongylus axei and T. colubriformis

Trichostrongylus axei and T. colubriformis infective third-stage larvae were frozen and stored for varying periods at -170 C: 10 to 80 days for T. axei and 10 to 38 days for T. colubriformis. Thawed, surviving larvae were used to infect rabbits. Survival percentages changed little between worms stored for the period of the test. Larvae surviving freezing, storage, and thawing were as infective ...

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Trichostrongylus colubriformis: relationship between ageing of infective larvae, infectivity and egg production by adult female worms.

Relationships between egg production and infectivity were studied with infective larvae of Trichostrongylus colubriformis which had been stored for up to 22 weeks at 24 degrees C. After different storage times, larvae were administered to lambs and the number of parasites which reached the adult stage was counted five weeks after infection. Egg production was estimated for the same batches of a...

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Relationship between exsheathment and enzyme activity (alkaline phosphatase and leucine amino peptidase) during ageing of Trichostrongylus colubriformis infective larvae.

A stock of Trichostrongylus colubriformis infective larvae (L3) was stored for up to 15 weeks at 24 degrees C. At three weeks intervals, samples of L3 were taken to assess exsheathment rate and enzyme activity Leucine Amino Peptidase (LAP) and Alkaline Phosphatase (AP). From 0 to 9 weeks of storage, a decrease in enzyme activity was observed correlated with a decrease in exsheathment rate (perc...

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ژورنال

عنوان ژورنال: PLoS Pathogens

سال: 2009

ISSN: 1553-7374

DOI: 10.1371/journal.ppat.1000597